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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of the gene has become observed in several tumors, which leads to chromosome instability, and thus may contribute to tumorigenesis. This protein was found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to your ATM locus), which participates in cell-cycle regulated histone gene expression and plays a crucial role in endorsing cell-cycle development from the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, are already explained. Two extra splice variants had been noted but specific nucleotide sequence details is not nonetheless accessible.

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Blocking peptides are peptides that bind precisely into the target antibody and block antibody binding. These peptide generally is made up of the epitope identified with the antibody.

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This tutorial outlines further more doable optimization of mobile and immunoassay parameters to guarantee the absolute best final results are...

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This guide outlines additional possible optimization of mobile and immunoassay parameters to ensure the very best effects are...

Antibodies sure to the blocking peptide no more bind on the epitope within the concentrate on protein. By comparing the staining from the blocked antibody as opposed to the antibody by itself, one can see which staining is particular.

Target Information Cyclin E belongs for the very conserved cyclin household, whose customers are characterized by a remarkable periodicity in protein abundance throughout the cell cycle. Cyclins purpose as regulators of CDK kinases. Distinct cyclins exhibit distinctive expression and degradation styles which contribute for the temporal coordination of each and every mitotic event. Cyclin E types a posh with and capabilities as being a regulatory subunit of CDK2, whose action is necessary for mobile cycle G1/S transition. This here protein accumulates with the G1-S phase boundary which is degraded as cells progress by way of S phase.

The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by equally antibodies...

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